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KMID : 0811720100140040235
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 4 p.235 ~ p.240
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Toll-like Receptor3-mediated Induction of Chemokines in Salivary Epithelial Cells
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Li Jing-Chao
Jeong Mi-Young
Bae Ji-Hyun
Shin Yong-Hwan
Zhu Mei-Hong
Hwang Sung-Min
Lee Jeong-Chai
Lee Sung-Joong
Park Kyung-Pyo
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Abstract
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Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic: polycytidylic acid (poly(I:C)) significantly increased interferon-?-inducible protein 10 (IP-10), interferon- inducible T-cell ? chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(I:C)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(I:C) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells.
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KEYWORD
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Toll-like receptors, HSG cells, Chemokine, Poly(I:C), IP-10
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